I've analyzed my RNA-seq data using DESeq2 and now I am trying to see whether some gene of interest are expressed in our dataset. I found this paper which uses RPKM cut off of 0.3 to defined genes as expressed. I found out that I can use the fpkm function in DESeq2 to convert the counts to fpkm/rpkm. However, reading some posts in here, it seems that rlog or vst normalized counts are recommended instead. Are there any common rule of thumb for the rlog or vst-normalized counts to define expressed genes? Thanks in advance!