Hi all,
I need a little bit of help. I am currently working on the secondary analysis of RNAseq data, and I cannot use an HPC. Some of the data that I need to work on are from The database of Genotypes and Phenotypes (dbGaP). I am still waiting for my Pi to get access to the data of 600 patients, but in the meantime, I am working with SRAs. Do you recommend any platform that I can use instead of an HPC? I am thinking about cloud computing, but I have no idea what service to use and how much computational power purchases to have decent speed for using software such as Kallisto.
Thank you in advance, and have an awesome day!
Best, Diana
Well, actually Kallisto can be ran on your laptop in notime, that's its entire purpose. Check out deploit https://deploit.lifebit.ai/app/home or firecloud by the Broad https://software.broadinstitute.org/firecloud/ they both supply an abstraction layer between your needs and the cloud. The process you should probably go through is defining a pipeline, either use an available one or build your own and then you can try and run this same pipeline in the cloud through these websites in an easy(er) way.