Question: Deferentially Expressed Genes using Genes count data obtained from RNASeq
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gravatar for naseerkhan861
6 weeks ago by
naseerkhan8610 wrote:

I have obtained Genes Count data after the analysis of RNASeq dataset , Now I want to analyze my dataset and extract DEGs(Differentially Expressed Genes) as my end goal is to perform clustering using WGCA once I have the DEGs from my dataset, Can somebody guide me how to proceed or guide me any link that I can follow accordingly. Also is it a good way to proceed like this I mean first finding DEGs and then do WGCNA?

Regards

rna-seq genescount degs • 108 views
ADD COMMENTlink modified 6 weeks ago by Benn7.9k • written 6 weeks ago by naseerkhan8610
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gravatar for Benn
6 weeks ago by
Benn7.9k
Netherlands
Benn7.9k wrote:

A good way to start is reading the manual of limma. Read about the RNA-seq methods and follow some of the case studies. Good luck.

ADD COMMENTlink written 6 weeks ago by Benn7.9k

Thanks Benn for your reply, I am reading limma, Thank you very much indeed!

ADD REPLYlink written 6 weeks ago by naseerkhan8610
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