I have two single WES from a child and his father. I would like to create a set a of rare variants inherited from the father. Afterwards, my goal is to compare if these rare variants still be found in a WES where I have pooled 2 fathers, to tests the sensibility of pooling.
I have been told that I could use Tabix but I don't know where to find the information on allele frequency in general population from GnomAD, to see what variants are rares? I could work on FastQ, cram and VCF formats.