Entering edit mode
4.5 years ago
Akshaya
•
0
Hello ! I downloaded multiple run accession files (RNAseq-FASTQ) from ENA and tried to concatenate them using cat.
cat *_1.fastq.gz > merged_R1.fastq.gz
The output file is bigger in size, however, when I look at the read counts it is the same as the first of all the files that were concatenated. I am a beginner in NGS data analysis. Can someone please advise what am I doing wrong/missing.
Thank you
What is
${i}
? Please show the entire code including how you determined the read number.I used the readlength.sh script in bbmap
I had the files for different samples in individual folders.
Looks fine, did checking read counts with the suggestion from RamRS help? I guess everything is fine and simply the counting went wrong.
Yes, RamRS's suggestion did help. Thank you