Question: Minfi preprocessNoob vs preprocessQuantile normalization for Illumina EPIC array
gravatar for jeni
3 months ago by
jeni0 wrote:


I would be very grateful if someone could explain me which method is better to use for differencial methylation analysis of 2 groups of samples from the same tissue (the difference is that one group respond and the other don't to a treatment). Would it be better to apply Noob or Quantile normalization? and what is the reason for that?

Thanks in advance.

minfi bioconductor R • 143 views
ADD COMMENTlink modified 3 months ago by zx87549.1k • written 3 months ago by jeni0

Any reason why you don't think other preprocessing normalization options in minfi (SWAN or Functional normalization) could be even better?

ADD REPLYlink written 3 months ago by igor9.9k

Thanks for your response. Actually I am not sure about what to use; but what I undestood from what I read is that Noob and Quantile are the more appropiate methods for closer groups, while the others are better for globally different groups.

ADD REPLYlink modified 3 months ago • written 3 months ago by jeni0
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