Entering edit mode
4.3 years ago
Dr.Animo
▴
130
Hi everyone, I am trying to perform DEG analysis. I've two samples each has 3 replicates (one is XR and other is YR) replicates are XR1,XR2,XR3 and YR1,YR2,YR3..
d<- DGEList(counts=as.matrix(raw.data[,2:7]), lib.size=library.sizes, group=expt)
d<- calcNormFactors(d)
At the following step, I got a warning message
d<- estimateCommonDisp(d)
Warning message:
In estimateCommonDisp.DGEList(d) :
There is no replication, setting dispersion to NA.
d$common.dispersion
[1] NA
How to resolve this?
