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• Using lima for Pacbio iso-seq data
  Hi, I just got back some Pacbio isoseq data. So I sequenced my sample on two different cells. Bo...
• pbalign and SMRT reads
  Hello, I recently used exonerate to align PacBio Iso-Seq SMRT reads. Although this worked, it wa...
• Use PacBio's iso-seq data to look for variant
  I am trying to identify A to I RNA modification in human genome. I have a list of potential modif...
• PacBio end-to-end pipeline advise
  Dear all, Could you please advise an end-to-end PacBio pipeline if available? There are too man...
• How to do pacbio iso-seq analysis begin with NCBI SRA fastq data
  Hi, I have downloaded some public data **PRJNA427246** from SRA database. The paper is “Hybrid ...
• PacBio coverage question for a plant genome
  Hi all, Can anybody suggest how much pacbio coverage do we need for a genome of 1.7 giga bases (...
• HGAP using old and new type of PacBio data
  I have a few PB runs on the same organism but in new (bax.h5) and old (bas.h5) format, presumably...
• how to get the error rate of iso-seq data?
  how about the error rate of iso-seq data? what can we do to improve the analysis of it?
• Transcript length distribution plot, based on genome + annotation
  Hi everyone! I am really wondering, with which tool is it possible to make a plot of transcript ...
• TAMA: long read RNA sequencing (Iso-Seq and Nanopore) processing tools
  [TAMA][1] (Transcriptome Annotation by Modular Algorithms) was designed for processing Iso-Seq da...
• How create annotation file for WGCNA for RNA-Seq data?
  Hello Biostars, I am currently working on WGCNA analysis for RNA-Seq data, the input gene count m...
• Adding asterisks to a ggplot2 bar plot to show significance
  Hi guys. I'm working with a data set and I've written a for loop that generates barplots for my i...
• How to find fusion gene using iso-seq?
  I would like to find some fusion genes using iso-seq method. Is there any tutorial about the pipe...
• Should I Join broken pacbio sequences and use them to scaffold Contigs ?
  Hi folks, I received pacbio sequences produced by sequel system and wish to use the sequences fo...
• ChIPSeeker Gene ID not matched error
  Hi I am using the ChIPSeeker package to do gene annotation for ChIP-seq data. broadpeak files wer...
• Blasr PacBio aligner: Unexpected sam file output
  I am aligning some PacBio single read sequences using Blasr. I was able to successfully align and...