I'm extracting positions from a gVCF using a bed file with break_blocks, with positions like
break_blocks --region-file $bed --ref human_g1k_v37.fasta --exclude-off-target
and then GATK's selectVariants:
gatk SelectVariants -R human_g1k_v37.fasta -V $vcf -O $out_vcf --remove-unused-alternates
to produce the following output:
1 1273477 1273478 seq-rs797044834 . GGGGGCAGCCGGGT G PASS . GT:GQ 0/0:0.29906204
and a VCF generated from break_blocks & GATK with lines like
1 1273478 . G . . . AN=2;DP=40 GT:DP:MIN_DP 0/0:40:35
the lack of ID & ALT is obviously not acceptable.
However, the problem is with the REF position, as it has mysteriously switched places. This will throw off the integer in the GT position.
Why did the REF switch places with the ALT? How can I fix it?
