I'm running an RNA-Seq analysis for which I have 72 samples, (6 replicates for each observed group). Using voomWithQualityWeights() instead of voom() returns a lot more diferentially expressed genes. I was wondering how can I know if I should use voomWithQualityWeights() or not?
I understand it has to do with outliers. I only have on 2 samples that seem to be outliers on the MDS plots. But as I have 72 samples I think it would be ok to simply remove them and run the analysis with regular voom(). Is there any way to test which voom method I should use?
Now asked on Bioconductor: https://support.bioconductor.org/p/129039/