Hi i have a quick question, i have few aligned bam files from single cell RNA Seq data. I want to regenerate fastqs from them. In order to do so i am using cellranger's bamtofastq and I am also getting fastq files but in the specified path within a folder named “MissingLibrary_1_flowcellName”. I am not sure what does this mean? Why the generated folder is named as missing library. Does anybody have any idea about this?
The command I used is:
cellranger bamtofastq possorted_genome_bam.bam ./sample
Any help will be highly appreciated.