Question: Logit-normal distribution to model variation among biological replicates
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gravatar for CY
8 months ago by
CY550
United States
CY550 wrote:

I came across rMATS while doing research on differential alternative splicing (AS). It uses logit-normal distibution to model variation among biological replicates. I am having difficulty understanding the logics here.

Can anyone share some insights on how logit-normal distribution can model such variation? It does not have to be AS specific as long as the allow me to understanding the rational here.

ADD COMMENTlink modified 8 months ago by Kevin Blighe67k • written 8 months ago by CY550
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gravatar for Kevin Blighe
8 months ago by
Kevin Blighe67k
Republic of Ireland
Kevin Blighe67k wrote:

It says, in the paper:

rMATS uses a binomial distribution to model the read count from the exon inclusion isoform given the exon inclusion level in each individual replicate and a logit-normal distribution to model the variation among replicates within sample group.

This is a big assumption that likely does not hold for all genes, which is a limitation of rMATs. I guess that they mean, that, if you plot the read counts for every exon over every identified isoform, then it would follow a binomial. Exons that are only expressed in rare isoforms would be at either tail of the curve.

ADD COMMENTlink written 8 months ago by Kevin Blighe67k

Thank you for replying. I can understand the binomial distribution part. What confused me is the logic of using logit-normal distribution to model between-replicates variation

ADD REPLYlink written 8 months ago by CY550
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