Entering edit mode
4.1 years ago
Hey everyone,
So I have used bcftools "mpileup" in combination with the multi-allelic caller to discover mutations in desired bacterial RNA samples. Here is some data given out by the bcftools.
Now comparing the sequences of these genes:
So basically, two each of the genes in the first image have the same sequences. I am wondering, how bcftools is able to differentiate between the two genes that share same transcripts. I am asking such a question because it is giving me two different qual values for two genes with the same sequence.
Can anyone shed some light?
Thank you