cellRanger 2.0 to Seurat 3.1.2
2
0
Entering edit mode
4.0 years ago
cge ▴ 20

Hi, anybody know if data from cellRanger 2.0 are still readable with Seurat 3.1.2? I am getting an error, I saw several threads but still no luck. Thanks S.

RNA-Seq software error • 2.2k views
ADD COMMENT
0
Entering edit mode

Yes, it is. Please add details. "I am getting an error" is not a reproducible error description. Show code and exact error messages please.

ADD REPLY
0
Entering edit mode

Hi, thank you for you answer. Here the code and error:

mat <- Read10X(data.dir = dataDir)
Error in dimnamesGets(x, value) :  
 invalid dimnames given for “dgTMatrix” object
In addition: Warning messages: 1: In readLines(barcode.loc) : line 15647540 appears to contain an embedded nul
2: In readLines(barcode.loc) : incomplete final line found on '/data/barcodes.tsv'
ADD REPLY
1
Entering edit mode
4.0 years ago
igor 13k

As the error says, you have an incomplete line. That probably means that there was an error in transferring the file.

ADD COMMENT
0
Entering edit mode
4.0 years ago
cge ▴ 20

Thanks! I downloaded again the file and it worked. S.

ADD COMMENT

Login before adding your answer.

Traffic: 2842 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6