Hi everyone, i would like to ask for help with this issue. Im really new in bioinformatics. I performed a DE analysis, and i get my gene list for example
GeneID baseMean log2FoldChange lfcSE stat pvalue padj Protein Name EGR_00013 64.5598042715165 2.00136220603475 0.231578525192148 8.64226164483152 5.51110427599768e-18 8.66468061170746e-17 hypothetical protein EGR_00013 EGR_05826 185.049060341855 2.00457061223278 0.223024449591272 8.98812043211619 2.5149436651832e-19 4.6174815794538e-18 Focal adhesion kinase EGR_04778 580.609080320372 2.00638021821051 0.157821469392399 12.7129738807713 5.00925473945179e-37 4.11109536466808e-35 calcium-binding protein
Now i would like to do an GO analysis, and i think my first step should be get the GO terms.
In omicsbox i may see the feature of Functional analysys and BLAST2go anotation.
My questions are:
Is that Pipeline right? (get Go terms by go anotation, then perform an enrichment analysys and finally get the charts)
How do i load my genes to blast2go to perform this analysis? i tried a .txt file with all columns (geneid, basemean, etc) and even with only geneID but the options doesnt appear for selection...
Please be patient, im a veterinary! thank you!