Hello all, I am new to de novo assembly and working with sequencing data in general. I am working with some paired end sequences. I just ran them through trimmomatic to remove adapters and am left with the forward and reverse paired sequences, and the forward and reverse unpaired sequences. When running trinity for de novo assembly, would it be the best practice to just assembly using the paired sequences for all samples I am analyzing, or should I include the unpaired sequences as well? What would be the pros or cons of each?