I am trying to identify which genes are significantly variable in four groups. How I was doing this was through:
Samples_results<- results(Samples_dds, alpha = 0.05, lfcThreshold = 1)
Which then provided me a list of a few hundred genes which were over the threshold. However, I am not sure if this was the correct way of testing this, and I was wondering if anyone had any advice?
Thanks!