FastQC error (failure to process fastq.gz file)

Question

[mdmoandd]

I was wondering why I got this error when using fastqc. Do I need to unzip my fastq.gz file first maybe?

Started analysis of 16_2.fastq.gz

Approx 5% complete for 16_2.fastq.gz

Approx 10% complete for 16_2.fastq.gz

Approx 15% complete for 16_2.fastq.gz

Approx 20% complete for 16_2.fastq.gz

Approx 25% complete for 16_2.fastq.gz

Approx 30% complete for 16_2.fastq.gz

Approx 35% complete for 16_2.fastq.gz

Approx 40% complete for 16_2.fastq.gz

Approx 45% complete for 16_2.fastq.gz

Approx 50% complete for 16_2.fastq.gz

Approx 55% complete for 16_2.fastq.gz

Approx 60% complete for 16_2.fastq.gz

Approx 65% complete for 16_2.fastq.gz

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Failed to process file 16_2.fastq.gz
uk.ac.babraham.FastQC.Sequence.SequenceFormatException: Midline '@A00817:68:HK7MFDRXX:1:2252:17409:11506 2:N:0:CTTGTCGA+GAACATCG' didn't start with '+'
    at uk.ac.babraham.FastQC.Sequence.FastQFile.readNext(FastQFile.java:172)
    at uk.ac.babraham.FastQC.Sequence.FastQFile.next(FastQFile.java:125)
    at uk.ac.babraham.FastQC.Analysis.AnalysisRunner.run(AnalysisRunner.java:77)
    at java.base/java.lang.Thread.run(Thread.java:834)

Answer 1

[GenoMax]

No you do not need to unzip the file to run FastQC. Looks like you have a corrupt fastq record. You may need to re-download the file.

[JC]

yes, looks like it's a corrupted file, hopefully you have a copy