Paired sample RNA-seq dataset analysis

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3.9 years ago

pedram • 0

Hi everyone,

I need to extract paired (normal vs cancer) RNA-seq data from any dataset. Then find common significant genes between cancer and normal (my gene of interest will fall among significant genes) Next, sort significant genes that most probably their expression is influenced by my gene of interest.

Can you please provide me very detailed workflow and even some personal help. I never done bioinformatics analysis and I truly appreciate anyone who can help me here.

Thanks a lot.

RNA-Seq • 640 views

ADD COMMENT • link 3.9 years ago by pedram • 0

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If you never did any RNA-seq then please start with https://www.bioconductor.org/packages/devel/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html and the many online resources available via google.

ADD REPLY • link 3.9 years ago by ATpoint 82k

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can you please share with me which online dataset I can use to export RNA-seq data. I use following link http://firebrowse.org/?cohort=LUAD&download_dialog=true but the data is not paired it is only lung cancer patients, and there are different formats any idea ?

ADD REPLY • link 3.9 years ago by pedram • 0

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