Question: Make a reference transcriptome for nonmodel organism
gravatar for abantika2005
4 weeks ago by
abantika20050 wrote:

I am planning to identify SNP within some strains of a nonmodel organism. The organism doesn't have the whole transcriptome sequence identified. So my plan is to make one of the strain's RNA sequence as a reference transcriptome sequence. I have completed the de Novo assembly and now I have around 2000 transcripts(or you can say contigs) for one strain. Can anyone advise me how can I join these transcripts (or RNA contigs) to make a reference RNA sequence?

Thanks in advance!

R rna-seq alignment assembly • 96 views
ADD COMMENTlink modified 4 weeks ago by ugurcabuk110 • written 4 weeks ago by abantika20050
gravatar for ugurcabuk
4 weeks ago by
ugurcabuk110 wrote:

the link I shared you can check de novo transcriptome assembly workflow

ADD COMMENTlink written 4 weeks ago by ugurcabuk110
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