Which fasta files are assembled transcripts IDBA_tran assembler ?

Question

[sunnykevin97]

Hi

I used IDBA_tran denovo assembler for RNA_seq assembly. After assembly I generated lot of files ? 1)contig.fa for 20,30,40,50,60 kmer's , filesize - 115M,...120M 2)transcript.fa for 20,30,40,50,60 kmer's, filesize - 49M ...89M which fasta files are considered to be assembled transcripts ?

Please let me know, I suppose no information was given in the manual.

suggestions please.

Answer 1

[weidonglu]

It should be transcript-20.fa,transcript-30.fa,transcript-40.fa,transcript-50.fa and transcript-60.fa. Then you have to merge these fasta-files for further analysis.