Is this a normal mean/variance scRNA plot?
0
0
Entering edit mode
2.1 years ago
aaragak1 ▴ 40

I've been following bioconductor's OSCA book for some scRNAseq analysis (smart seq2 protocol, no spike-in). After QC, normalization (deconvolution) and transformation (log2, pseudocount 1) upon plotting the mean/variance of the genes, I noticed that the plot looked very much like a negative parabola: mean_var

This isn't what I'd seen in the OSCA examples. I was wondering if this is a cause for concern, or if I should continue forward into feature selection.

Thank you for your time! Let me know if I should provide any more information.

RNA-Seq R • 764 views
ADD COMMENT
0
Entering edit mode

Can you share the code please?

ADD REPLY

Login before adding your answer.

Traffic: 1280 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6