I am very new to blast+. I tried to create a database from my own sequences (not downloading existing databases). The sequences are genomes. I tried to look at manuals how to do it but could not find the answer. This is what I got:
C:\Program Files\NCBI\blast-2.10.1+\bin>makeblastdb -in 1genome.fasta -dbtype nucl -out 1genome Building a new DB, current time: 06/22/2020 12:40:54 New DB name: C:\Program Files\NCBI\blast-2.10.1+\bin\1genome New DB title: 1genome.fasta Sequence type: Nucleotide Keep MBits: T Maximum file size: 1000000000B No volumes were created. Error: mdb_env_open: Input/output error
Are there any mistakes? Also I'm wondering, is it possible to create a database from a singe sequence?