Entering edit mode
3.7 years ago
akashbala0
▴
10
Hi everyone,
I have aligned my RNAseq data in hisat2 and I am confused with the Alignment Result.
31749920 reads; of these:
31749920 (100.00%) were unpaired; of these:
199326 (0.63%) aligned 0 times
28419292 (89.51%) aligned exactly 1 time
3131302 (9.86%) aligned >1 times
99.37% overall alignment rate
As I'm working on DEG analysis I have 9.86% aligned more than one time. Is anything wrong with processing these data, can I proceed with this data.
why do you think this is not a valid result to continue for a DEG analysis?
Because 9.86% are aligned more than once.
and this for educational purposes :)
why do you think you need the ones that align more than once? (do you fully understand what that means?)
akashbala0 : Please do not delete threads that have received comments/answers. Deleting posts is not the way to indicate that a problem was solved. Accepting answer(s) is. You have already done that. Thanks!