I am trying to do a de novo transcriptome with paired-end reads using Trinity. I have received the following error:
pairs.K25.stats is empty. Be sure to check your fastq reads and ensure that the read names are identical except for th
e /1 or /2 designation. at /usr/local/trinity/trinityrnaseq-Trinity-v2.4.0/util/insilico_read_normalization.pl line 904.
CMD finished (38 seconds)
My files are quite large and I'm using quite a few, I was wondering if anyone had any advice on how to identify where this error is originating from without manually scanning all of my files
You should have used a utility like
repair.sh
from BBTools. It is not practical to manually inspect things like large sequence data files.