Question: Methodology: Meta-analysis and integration of published transcriptomics and proteomics data
gravatar for Cookie-san
3 months ago by
Cookie-san100 wrote:

Hello everyone!

I would like to compare DEGs across transcriptomic datasets and compare them with DEGs across proteomic datasets available for the same disease condition. Are there any standard protocols to follow for this specific purpose?

  1. I have transcriptomics datasets from different tissues but for the same condition. Should I start with raw data and re-analyze the DEGs and then compare, or is there any other method I can follow?

  2. Should I compare data from the same tissues after DEG together or can I compare from all tissues?

  3. For proteomics data, the papers have given the DEG protein list. Again, should I start from raw data or use the list? What are the best parameters to consider? Like eg. MIST, fdr-p value, fold change?

  4. How can I compare the DEGs from different transcriptomics datasets? Should I use a statistical test?

  5. After meta-analyzing transcriptomics data and arriving at one single list of DGE genes, and similarly one meta-analyzed list of DGE genes from proteomics datasets, how can I correlate the data? Is there any statistical analysis or test I can follow?

If you can suggest me a paper or any material, even if it's a book, that'll be more than helpful!

Thank you so much, and if this post made you roll you eyes on how dumb it is, I'm sorry, its my very first time doing this kind of analysis and I simply can't find any proper review papers!

rna-seq omics proteomics • 164 views
ADD COMMENTlink modified 3 months ago • written 3 months ago by Cookie-san100

So I figured out the answer!

To compare any two gene lists you can do a hypergeometric test or a Fisher's exact test. I am using the R package GeneOverlap.

ADD REPLYlink written 8 weeks ago by Cookie-san100
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