Hello. I am a Bachelor student and working on fungal identification from rose bushes. I obtained around 60 samples and after DNA extraction the samples were sequenced through Sanger sequencing. As primer we used ITS1. Now I am blasting my results and I am not happy about some of the results. In general I would like to know what kind of tools or information do I need to obtain? Should I learn Perl? Which online tools or software tools do I need? And what type of books should I take a look into? I have so little knowledge that I dont even know what type of knowledge I need to obtain and in which order. Could someone tell me what skills I need to get if I want to handle sequences? And also which tools and sources would get me the skills?
Happy if someone could tell me a list and an order on how to learn more skills. Thank you, Oliver