Off topic:freebayes problem in sample name in vcf
0
0
Entering edit mode
3.7 years ago
evelyn ▴ 230

Hello,

I am using following command to create a vcf file with freebayes:

freebayes  -f  transcripts.fasta duplicates.bam --max-complex-gap         1 \
          --theta                   0.01 \
          --ploidy                  2 \
          --min-alternate-fraction  0.2 \
          --min-alternate-count     2 \
          --min-coverage            3 \
          --min-mapping-quality     1 \
          --min-base-quality        20 \
          --report-genotype-likelihood-max \
          --throw-away-complex-obs \
          --throw-away-mnps-obs \
          --throw-away-indel-obs > SNPs.vcf

I created the bam file using:

bwa mem transcripts.fastaR1_paired.fastq.gz R2_paired.fastq.gz > paired_bwa.sam
samtools view -S -b paired_bwa.sam > paired_bwa.bam
samtools sort -o paired_bwa.bam -O bam paired_bwa.sorted.bam
samtools index paired_bwa.sorted.bam

but the vcf file does not show sample name in the header, it shows unknown:

#CHROM  POS ID  REF ALT QUAL    FILTER  INFO    FORMAT  unknown

I want the sample name to show instead of unknown. Thank you!

snp • 687 views
ADD COMMENT
This thread is not open. No new answers may be added
Traffic: 2105 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6