Problem with file location address in HISAT2
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Entering edit mode
8 months ago
yoosefyud ▴ 40

Hello dear Biostars community I was trying to align my sequence with hg-19 genome reference using HISAT2. My system is dual boot with windows 10 and Ubuntu. so, when i want to give my file address i get the following error:

./hisat2 -q -t -p 8 --add-chrname -x /media/yousef/EEFC0BDBFC0B9CC9/2.Medical labratory scineces/Genetice/bioanformatics/NGS/hg19_genome/genome /home/yousef/ncbi/public/sra/SRR6040558_Trimmed.fastq -s /home/yousef/ncbi/public/trimmed.sam
(ERR): "/media/yousef/EEFC0BDBFC0B9CC9/2.Medical" does not exist
Exiting now ...


And my file location is:

/media/yousef/EEFC0BDBFC0B9CC9/2.Medical labratory scineces/Genetice/bioanformatics/NGS/hg19_genome/genome


I also need to address that i have no folder named "EEFC0BDBFC0B9CC9" in my drive. Also,The trimmed FASTQ file that i'm trying to use is not in the same drive that my Ubuntu is installed. How can i solve this problem?

next-gen sequence RNA-Seq genome software error • 319 views
1
Entering edit mode
8 months ago
ATpoint 49k

Whitespaces have to be escaped in bash. Best is to either remove them from the folder name or if this is not possible type

realpath path/to/genome

and copy the output.

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Entering edit mode

Thanks a lot. It solved my problem.