I am trying to perform a differencial expression analysis between two groups of patients. But I would like to group them taking into account two variables.
Imagine I have these data:
patient1 T1 6 patient2 T1 2 patient3 T4 2 patient4 T2 0 patient5 T3 1 patient6 T2 4 patient7 T3 5 patient8 T1 0
Would it be possible to group patients in two groups (for example:
late) taking into account both variables? As an example: early patients could be those presenting
T2 status referring to the first variable and a value shorter than 4 referring to the second variable. I wouldn't like to be subjective, so I would like to use some program which could perform this grouping.
On the other hand, would it be possible to create a design matrix with the two variables and then use
limma to perform the differencial expression analysis taking into account both variables to group patients?