mitochondria in single nuclear RNAseq
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Entering edit mode
13 months ago

for snRNAseq, do people use mitochondrial filtering? I'm not sure if this still makes sense even though we are working with data from nuclei rather than whole cells, but I do see some expression of mito genes in my data.

Also, is it preferable to filter the cells based on mito content or to regress it out?

snrnaseq scrnaseq seurat single cell RNAseq • 443 views
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