Entering edit mode
3.5 years ago
zinwinlatt14
•
0
Hello guys!
I'm newbie to Bioinformatics platform. Actually, I was using Trinity for De novo transcriptome assembly with following scripts and then, I got errors (please kindly find the attached file). It doesn't produce any output and I can't proceed to the next step. Could you please help me to fix this issue? Thank you.....
I've done trimming step and combine all paired forward/reverse reads into single file.
zcat /home/zwl/02trim/_P_1.fastq.gz > fw.fq
zcat /home/zwl/02trim/_P_2.fastq.gz > rv.fq
~/miniconda3/envs/bioinfo/bin/Trinity --seqType fq --SS_lib_type RF
--no_version_check
--left fw.fq
--right rv.fq
--CPU 60 --max_memory 800G
1>job.out 2>job.err
