I have two PromethION libraries, one standard, and the other with ultralong read chemistry.
To create a draft assembly i pooled the fastqs of both libraries and assembled from the result.
Now i am coming to nanopolishing the assembly and it requires the fast5 signal files of reads used in the assembly, which are still seperate and unpooled.
Can i just pool the fast5s? This seems difficult due to nanopolish needing the
sequencing_summary.txt file, of which there is a separate one for each library.
Should i not have pooled the fastqs for assembly, and instead just use the ultralong readas to scaffold?