Low DESeq2 Sensivity on scRNA Pseudobulk Samples

Question

[Frenkiboy]

I'm encountering an unexpected problem when running DESeq2 on pseudobulk scRNAseq datasets. I have three replicates per condition, and although some genes show evident differential expression, DESeq2 gives them extremely high p values. These genes do have low expression, but the size effect is quite reproducible.

All of the standard diagnostic plots look OK (mean - variance fit, heatmaps, PCA).

Any ideas how to increase the sensitivity of the test would are welcome.

[ATpoint]

Can you show both the stats of this gene and where it is located on a MA-plot? Is it a low-count gene?