Problem with recognizing adapters while using Cutadapt or Trimmomatic
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3.4 years ago

I got my samples sequenced from a commercial facility. They used the following adapter sequences for sequencing

Sample ID   Index i7            Index i5
S0A         GTTCTTCTGT  CCAAGGTATT
S0B         CCAAGGTATT  GTTCTTCTGT
S0C         TGTGGAGAGG  AACGAACCGG
S5A         TTCCTTGAGG  TGCTGTCTTA
S5B         GTTAATTGCG  GAGGAGATAA
S5C         GAGGAGATAA  GTTAATTGCG
S15A    GTTCCTCATT  AATGTCGGTT
S15B    AATGTCGGTT  GTTCCTCATT
S15C    TTGACAGGTA  TGGTGGTTGT

I am confused with the nomenclature of adapters. I am trying to use the Cutadapt tool in galaxy, and I am not sure out of the given adapter sequences which are 3'(End) adapters, 5'(Front) adapters, or 5' or 3' (Anywhere) Adapters. I also tried using the trimmomatic tool but I am not sure if I should use the TruSeq2 (pair ended) or TruSeq3 (pair ended). Could anyone please guide me through this so that I can start my data analysis? Thank you.

RNA-Seq • 638 views
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Please use the formatting bar (especially the code option) to present your post better. You can use backticks for inline code (`text` becomes text), or select a chunk of text and use the highlighted button to format it as a code block. I've done it for you this time.
code_formatting

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Thank you. I will take care of it next time.

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