Entering edit mode
3.3 years ago
nkabo
▴
80
Dear community,
I have 2 FASTQ files from 2 samples and I have been working on finding which unspliced tRNAs are present in these 2 files coming from these samples. I am not sure whether an unprocessed tRNA would be visible in a FASTQ file (since I consider that it could be evaluated as an artefact), @swbarnes2 suggested me to obtain sequences of unspliced tRNA genes and do the aligning afterwards.
However, I could find regular tRNA sequences, but I could not see any groups of sequences, specifically belonging to unspliced tRNAs.
Could you suggest me a way to find sequences of unspliced tRNA genes?
Thanks in advance!
