6.6 years ago by
The Trinity tool for RNA-Seq has a component called "Chrysalis" that generates deBruijn graphs. If you visit the link I provided, you can see how to run Chrysalis in isolation, which may give you what you want. Digging deeper, there's a dedicated tool used by Chrysalis called FastaToDeBruijn that might be exactly what you're looking for. I've never run that specific tool in isolation, but given what i know about the Trinity toolset and how it's assembled, I bet if you picked through the source you could adapt it to work with non-RNAseq data.
I'm actually pretty interested to see what you come up with. Keep me posted because I might like to join in if you run into difficulties.