How to statistically determine if a mutation has an impact on expression individually for one patient
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8 months ago
jeni ▴ 50

Hi!

I am studying a group of cancer patients from which I have INDELs (from DNA fastq) and expression data (RNAseq), both from a tumoral and a normal sample for each one of them.

As I know some genes affected by potential pathogenic insertions or deletions, I would like to assess, for each patient, an effect of the alteration in its expression. As this analysis is performed individually for each patient, as these mutations are unique, I am not sure how to perform it statistically. I would like to compare the expression levels between tumour and normal sample for each individual patient. As the comparisson is between two samples, I cannot perform an usual differencial expression analysis.

How can I achieve this?

Thanks!

RNA-Seq variant calling • 293 views
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Statistics and n=1 are mutually exclusive. You would need at least some n to perform some kind of regression analysis.

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