Question: Tru Seq small RNA library prep kit
gravatar for Katie __
6 weeks ago by
Katie __10
Katie __10 wrote:


Could anyone help me determine if illumina Tru Seq small RNA kit is unstranded or stranded (first or second)?

On illumina support is says tru seq small rna libraries cannot be used for directional RNA sequencing but this post quotes a paper that states it produces strand specific libraries. I am using featureCounts for assigning reads and wanted to make sure I have the correct flag.

Thank you

ADD COMMENTlink modified 5 weeks ago • written 6 weeks ago by Katie __10

If Illumina support (the manufacturer) says it is unstranded they I would trust that rather than a random paper from 2012 which only addresses this in a minor sentence and without any further information.

ADD REPLYlink written 5 weeks ago by ATpoint46k

ok, thank you!

ADD REPLYlink written 5 weeks ago by Katie __10

I ended up emailing illumina support and they said

TruSeq Small RNA produces stranded libraries. I believe the FAQ refers to the fact that the kit should not be used to produce stranded mRNA libraries with larger inserts, like the ones produced via TruSeq Stranded mRNA.

ADD REPLYlink modified 5 weeks ago • written 5 weeks ago by Katie __10
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