Hi, I'm not sure if this is the right place to ask but... I'm working with RNA-Seq data of tetraploid wheat and I'm interested in comparing differences in expression between my DEG (calculated using DESeq) to their homeologs. As far as I can understand I cannot use DESeq counts for this because they are not suitable for within-sample comparison. I am not sure if I should use TPM instead and if I do use TPM how can I say something meaningful on the overall differences between treatments (parents and hybrids in my case)?. Thanks.
Question: Comparing homeologs gene expression in polyploid species
5 weeks ago by
alonziso • 0
alonziso • 0 wrote:
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