I have a quick question about DEG and p-value interpretation in RNA-seq data.
When I use edgeR to get DEGs, two values are reported: log2FC and p-value. As you may know, many researchers consider DEGs satisfying with |log2FC| > 0.58 and p-value < 0.01. Here is my questions:
1. p-value < 0.01 means "significance of difference" and |log2FC| means strength.. (am I right?).
2. When I see my data, there are half of genes showing |log2FC| < 0.58 around p-value 0.01 ! Do I interpret that p-value 0.01 is too large to select DEGs?
Thanks for your attention!