Question: Multifactor analysis in DESeq2
1
gravatar for a.basitkhan1990
12 days ago by
a.basitkhan199010 wrote:

Hello!

I have a data-set of tumors and I am checking to see if smoking causes a transcriptional difference. We have divided our sample into ever and never smokers.

This same set of tumors has 3 transcriptional subtypes: A, B, and C. I want to control for the differential amounts of these tumors within the ever and never smoker groups.

In my clinical data sheet I put into DESeq2 I have 2 columns: Smoking - Y or N Type - A, B, or C

I encoded this in DESeq2 as:

dds <- DESeqDataSetFromMatrix(countData = cts, colData = coldata, design= ~ Type + Smoking)

However, I have been asked to do DESeq2 within each subtype and compare the results across the 3. So, do DESeq2 in only Type A then repeat for Type B only and Type C only. Afterwards, I see which genes are present in all 3 groups.

The results are more interesting the first way as opposed to the latter.

My question is, which of these methods is mathematically more accurate?

Thanks!

rna-seq • 90 views
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