Why does KAAS use SBH for partial genomes?
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3.2 years ago
Dunois ★ 2.5k

Hi,

So the KEGG Automatic Annotation Server's landing page (https://www.genome.jp/kegg/kaas/) indicates that it uses bi-directional best hits (BBHs for annotating "complete" genomes, while recommending using single-directional best hits (SBHs) for annotating a limited gene set ("partial genome").

My question is: why is this the case? Does the underlying annotation algorithm know when a set of sequences it is receiving represents a "complete" genome as opposed to an "incomplete" one? From what I know of the BBH methodology, it is dataset agnostic, so I don't quite see why KAAS makes this somewhat odd recommendation.

Is there some set of guidelines for when BBHs can be used?

Your inputs would be much appreciated.

orthology blast reciprocal best hit annotation • 669 views
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