recommended minimum cell numbers to pseudobulk for DE comparisons between groups

Question

[Sentinel156]

Dear all,

I have not been able to find any commentary on this so far so hoping for some help here.

What is a reasonable minimum number of cells per group for pseudobulking or for performing differential expression/Marker gene identification using MAST etc. I see in the muscat code (https://rdrr.io/bioc/muscat/f/vignettes/analysis.Rmd) a 10 cell minimum is used by default but I couldn't find any data behind this in the paper. Is this reasonable statistically?

Furthermore, any thoughts on best practises when comparing groups with a large discrepancy in cell number (e.g. 100 cells vs 1000+ cells)

I'm looking for some data investigating this if possible

Thanks in advance,