Bam splitting index .sbi ?

Question

[quentin54520]

Hello all,

I used MarkduplicatesSpark and at the end i obtained a bam splitting index (.sbi). I search on internet but i don't really found what it is exactly and if it's really mandatory ?

Thanks in advance 😀

Answer 1

[Pierre Lindenbaum]

https://github.com/samtools/htsjdk/blob/master/src/main/java/htsjdk/samtools/SBIIndex.java

SBI is an index into BGZF-compressed data files, which has an entry for the file position of the start of every nth record.

it allows to split the job in n-parts for Spark

[quentin54520]

Thanks, but it's really mandatory? I mean if i use the option --create-output-bam-splitting-index=false

it's change something ?