Entering edit mode
14 months ago
Hi!
My professor has asked me to generate simulated RNA-seq data. Preferably a dataset that contains expressed transposable elements (TEs). I am new to bioinformatics but have strong programming skills. I know that I should do it using Polyester, but it doesn't have strong documentation. Also, I couldn't find any tutorials online. Is there any tutorial that teaches generating simulated RNA-seq data step by step?
Thank you for your attention.
There are multiple read simulators available:
What Ngs Read Simulators Are Available For Paired-End Data?
NGS reads simulation
I suggest you look at
ART
and perhapsrandomreads.sh
from BBMap.