How to generate simulated RNA-seq data
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14 months ago

Hi!

My professor has asked me to generate simulated RNA-seq data. Preferably a dataset that contains expressed transposable elements (TEs). I am new to bioinformatics but have strong programming skills. I know that I should do it using Polyester, but it doesn't have strong documentation. Also, I couldn't find any tutorials online. Is there any tutorial that teaches generating simulated RNA-seq data step by step?

Thank you for your attention.

RNA-Seq • 263 views
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There are multiple read simulators available:
What Ngs Read Simulators Are Available For Paired-End Data?
NGS reads simulation

I suggest you look at ART and perhaps randomreads.sh from BBMap.

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