Question: Alternate Transcripts Paralogs
7.4 years ago by
aj123 • 70
aj123 • 70 wrote:
I have a few doubts related to how the best way is to go about finding these:
- Finding unique regions when you compare various transcripts of a gene in one organism.
- Finding evidences for a particular transcript.
- Finding the % identity IN NCBI PROTEIN database for a particular sequence taken from uniprot. i.e i blast a protein sequence in uniprot database and get the paralogs for that along with percent identity. then i want to find the corresponding sequences for those paralogs in NCBI protein and find the %ID for my query sequence against those NCBI protein sequences. i tried ID mapping in uniprot and it gives me the corresponding IDs. but when i blast my query sequence against these IDs the %identity is way off from the %ID uniprot gives me.
any help/suggestions would be greatly appreciated.
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