Question: What Is The Minimal Sequencing Depth To Identify Long Non-Coding Rna In Cell Cultures?
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wim.trypsteen • 20 wrote:
We are planning to compare expression patterns of long non-coding RNAs among different conditions in cell culture experiments. This will be performed by transcriptome sequencing on an Illumina HISeq platform. For normal mRNAseq we usually combine up to six samples with barcoded adaptors to save costs. Yet this decreases the sequencing depth per sample. Does anyone know how many samples could be combined on a HiSeq lane to get adequate results of lncRNA expression, taking the cost of sampling into consideration?
