Question: Tissue-Specific Expression Of Proteins
0
gravatar for foxylady
6.7 years ago by
foxylady10
foxylady10 wrote:

I would like to compile a list of proteins highly expressed in a given tissue. What would be the best and most complete resource for this purpose? I'm mostly interested in human/mice/pig, and preferably healthy tissue.

Thanks in advance! /F

expression protein • 2.2k views
ADD COMMENTlink modified 4.2 years ago by Floris Brenk890 • written 6.7 years ago by foxylady10

One comment: there can be many different cell types in a tissue. If one of them is of particular interest to you, try to look if someone did a microarray/RNASeq experiment on FACS sorted cells, using a marker of your cell type coupled to GFP.
For example, you can use OMP-GFP mice (OMP = olfactory marker proteins) to extract olfactory sensory neurons from their surrounding tissue, and find proteins they express specifically.

ADD REPLYlink written 4.2 years ago by cyril-cros890
2
gravatar for JC
6.7 years ago by
JC8.0k
Mexico
JC8.0k wrote:

Besides Protein Atlas, if you are ok with transcript expression from microarrays and RNAseq for tissue enrichment, you can use BioGPS, GeneCards, RNAseq atlas, AlexaSeq, EMBL GXA and VeryGene. Many of these sites have the data available for download.

ADD COMMENTlink written 6.7 years ago by JC8.0k
2
gravatar for Floris Brenk
4.2 years ago by
Floris Brenk890
USA
Floris Brenk890 wrote:

www.gtexportal.org/

Nice graphically pleasing RNAseq data

ADD COMMENTlink written 4.2 years ago by Floris Brenk890
0
gravatar for Niallhaslam
6.7 years ago by
Niallhaslam2.2k
Dublin
Niallhaslam2.2k wrote:

The human protein atlas has pretty comprehensive coverage for human proteins in a variety of tissues: http://www.proteinatlas.org/

Though its more of a qualitative resource and therefore may not satisfy your need to identify only highly expressed (assuming you mean high abundance) proteins.

ADD COMMENTlink written 6.7 years ago by Niallhaslam2.2k
0
gravatar for Biojl
6.7 years ago by
Biojl1.6k
Barcelona
Biojl1.6k wrote:

If you are working with humans the bodymap dataset may interest you. I attach you the link to download the raw RNA-seq data from the 16 different tissues generated in the project.

http://www.ebi.ac.uk/arrayexpress/browse.html?keywords=E-MTAB-513&species=&array=&exptype[]=&exptype[]=&pagesize=25&sortby=releasedate&sortorder=descending&expandefo=on

Hope it helps!

ADD COMMENTlink written 6.7 years ago by Biojl1.6k
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