Question: Differance Between Uni-Tags , Contigs , Tags ,
1
gravatar for Amr
6.1 years ago by
Amr10
Amr10 wrote:

hello I read in papers that use NGS this expression and i want to know the meaning difference between uni-tags , contigs , tags ,Singletone

best regards

Amr

contigs sequencing • 1.7k views
ADD COMMENTlink modified 6.1 years ago • written 6.1 years ago by Amr10
3
gravatar for Josh Herr
6.1 years ago by
Josh Herr5.6k
University of Nebraska
Josh Herr5.6k wrote:

If you're talking about expression, the difference between these terms is: a contig is an assembled group of sequences which form a "contiguous" sequence (where the name comes from), contigs can be assembled together to form scaffolds. Singletons are typically sequences which are represented by a single read and are not found in duplicate in a sequencing experiment, although I've heard some people refer to singletons as single copy genes represented in expression studies (ambiguities in terminology drive me crazy!)

I have issues with the word "tag" in sequencing experiments, so I can't explain to you the difference between what someone is calling a "uni-tag" and a singleton. I have seen the use of "tags" in the literature lately as a word-for-word replacement for reads (amplicon and whole shotgun sequences). I can't stand it's use as a replacement for "reads" as it leads to confusion with actual sequence tagging techniques (like in a BAC clone) and is more ambiguous than the use of the type of read (amplicon, whole genome shotgun, marker-assisted selection, etc.).

ADD COMMENTlink written 6.1 years ago by Josh Herr5.6k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1858 users visited in the last hour